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AssigneeVib Vzw
InventorsCatherine Asleson Dundon, Christopher Smith, Piruz Nahreini, Johan Thevelein, Sofie Saerens
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The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the Saccharomyces clade, Crabtree-negative yeast microorganisms, Crabtree-positive yeast microorganisms, post-WGD (whole genome duplication) yeast microorganisms, pre-WGD (whole genome duplication) yeast microorganisms, and non-fermenting yeast microorganisms.
1-20. (canceled)
A recombinant yeast microorganism comprising enzymes of an isobutanol producing metabolic pathway, comprising at least one exogenous gene encoding a polypeptide that catalyzes a step in the conversion of pyruvate to isobutanol, wherein said recombinant microorganism is engineered to reduce the expression and/or activity of one or more of the endogenous transporter proteins encoded by a gene selected from AQR1 and PDR12.
Dependencies are inferred from the claim text.