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US 2016/0108433 A1Published application
Systems, apparatus, and methods for droplet-based microfluidics cell poration
AssigneeDuke University
InventorsRichard Fair, Andrew Madison, Matthew Royal
Life of this patent
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Abstract
The present disclosure provides systems, apparatus, and methods that include an electrowetting-on-dielectric microfluidic apparatus for effecting poration of cells in a droplet.
Claims28
An electrowetting-on-dielectric microfluidic apparatus for cell poration comprising:
- a first plate;
- a second plate spaced-apart from the first plate and defining a flow channel therebetween for receiving a droplet having cells therein;
- an acoustic energy generator in communication with the first plate for providing sonoporation to cells within a droplet received in the flow channel;
- and
- a resilient member in communication with the acoustic energy generator and in communication with a droplet received in the flow channel to provide acoustic streaming and sonoporation to cells within a droplet.
2 more elements
A system comprising:
- an electrowetting-on-dielectric microfluidic apparatus for cell poration that includes:
- a first plate;
- a second plate spaced-apart from the first plate and defining a flow channel therebetween for receiving a droplet having cells therein;
- an acoustic energy generator in communication with the first plate;
- and
- a resilient member in communication with the acoustic energy generator and in communication with a droplet received in the flow channel;
- and
- a control module configured for:
- controlling the acoustic energy generator to apply energy to the apparatus for providing acoustic streaming and sonoporation to cells within a droplet.
6 more elements
A method comprising:
- in a spaced-apart plate arrangement defining a flow channel therebetween, applying an acoustic energy field to a resilient member in communication with a droplet having cells therein to induce an ultrasonic vibration in the resilient member for providing acoustic streaming and sonoporation of the cells.
Dependencies are inferred from the claim text.