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US 2016/0108432 A1Published application
Droplet actuator for electroporation and transforming cells
AssigneeAdvanced Liquid Logic, Inc.
InventorsSrikoundinya Punnamaraju, Melissa A. Sandahl, John J. Harrington, Allen E. Eckhardt, Zhishan Hua, Michael G. Pollack
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Abstract
The invention provides a droplet actuator designed for performing electroporation on cells in droplets. The invention also provides method and systems for performing electroporation on cells in droplets on a droplet actuator.
Claims20
A droplet actuator comprising:
- a. at least one substrate, wherein droplet operations electrodes are associated with the at least one substrate;
- and
- b. one or more electroporation electrodes.
A droplet actuator comprising:
- a. a top substrate and a bottom substrate separated to form a droplet operations gap;
- b. droplet operations electrodes atop the bottom substrate facing the droplet operations gap;
- c. a via extending into the bottom substrate, whereby the droplet operations electrodes are electrically coupled to a power source;
- d. a dielectric layer atop the droplet operations electrodes and atop the bottom substrate in areas between the droplet operations electrodes;
- e. an electroporation electrode atop the dielectric layer, wherein the electroporation electrode comprises a footprint;
- and
- f. a hydrophobic coating atop the dielectric material surrounding the footprint of the electroporation electrode.
4 more elements
19-22. (canceled)
A method of producing electroporation, comprising:
- a. situating a droplet comprising cells atop an electroporation electrode in a droplet actuator, wherein the electroporation electrode is covered with a hydrophobic coating;
- and
- b. delivering a pulse to the electroporation electrode, thereby causing electroporation of the cells in the droplet.
24-55. (canceled)
Dependencies are inferred from the claim text.