Novel Chimeric Insecticidal Proteins Toxic or Inhibitory to Lepidopteran Pests
AssigneeMonsanto Technology LLC
InventorsJames A. Baum, Thomas A. Cerruti, Crystal L. Dart, Leigh H. English, Xiaoran Fu, Victor M. Guzov, Arlene R. Howe, Jay P. Morgenstern, James K. Roberts, Sara A. Salvador, Jinling Wang
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Abstract
Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.
Claims21
A chimeric insecticidal protein comprising an amino acid sequence as set forth in any of SEQ ID NOs:
- 21, 10, 28, 7, 4, 13, 16, 19, 23, 25, 30, 33, 36, 39, 41, 43, 45, 47, 50 or 53.
A transgenic plant cell, plant or plant part comprising a chimeric insecticidal protein, wherein:
- a. the chimeric insecticidal protein comprises any amino acid sequence set forth in any of SEQ ID NO:
- 21, 10, 28, 7, 4, 13, 16, 19, 23, 25, 30, 33, 36, 39, 41, 43, 45, 47, 50 or 53;
- or
- b. the chimeric insecticidal protein comprises a protein having:
- i. at least 94% identical to SEQ ID NOs:
- 21, 10;
- ii. at least 93% identical to SEQ ID NO:28;
- iii. at least 87% identical to SEQ ID NO:7;
- iv. at least 90% identity to SEQ ID NO:4;
- v. at least 91% identical to SEQ ID NO:13;
- vi. at least 64% identical to SEQ ID NO:16;
- vii. at least 66% identical to SEQ ID NO:19;
- viii. at least 86% identical to SEQ ID NO:23;
- ix. at least 91% identical to SEQ ID NO:25;
- x. at least 94% identical to SEQ ID NO:30;
- xi. at least 91% identical to SEQ ID NO:33;
- xii. at least 64% identical to SEQ ID NO:36;
- xiii. at least 66% identical to SEQ ID NO:39;
- xiv. at least 94% identical to SEQ ID NO:41;
- xv. at least 84% identical to SEQ ID NO:43;
- xvi. at least 93% identical to SEQ ID NO:45;
- xvii. at least 94% identical to SEQ ID NO:
- 47;
- xviii. at least 91% identical to SEQ ID NO:50;
- or
- xix. at least 93% identical to SEQ ID NO:53.
A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a chimeric insecticidal proteins, wherein:
- a. the chimeric insecticidal protein comprises any amino acid sequence set forth in any of SEQ ID NO:
- 21, 10, 28, 7, 4, 13, 16, 19, 23, 25, 30, 33, 36, 39, 41, 43, 45, 47, 50 or 53;
- or
- b. the chimeric insecticidal protein comprises a protein having:
- i. at least 94% identical to SEQ ID NOs:
- 21, 10;
- ii. at least 93% identical to SEQ ID NO:28;
- iii. at least 87% identical to SEQ ID NO:7;
- iv. at least 90% identity to SEQ ID NO:4;
- v. at least 91% identical to SEQ ID NO:13;
- vi. at least 64% identical to SEQ ID NO:16;
- vii. at least 66% identical to SEQ ID NO:19;
- viii. at least 86% identical to SEQ ID NO:23;
- ix. at least 91% identical to SEQ ID NO:25;
- x. at least 94% identical to SEQ ID NO:30;
- xi. at least 91% identical to SEQ ID NO:33;
- xii. at least 64% identical to SEQ ID NO:36;
- xiii. at least 66% identical to SEQ ID NO:39;
- xiv. at least 94% identical to SEQ ID NO:41;
- xv. at least 84% identical to SEQ ID NO:43;
- xvi. at least 93% identical to SEQ ID NO:45;
- xvii. at least 94% identical to SEQ ID NO:
- 47;
- xviii. at least 91% identical to SEQ ID NO:50;
- or
- xix. at least 93% identical to SEQ ID NO:53;
- or
- c. the polynucleotide segment hybridizes to a polynucleotide having a nucleotide sequence as set forth in any of SEQ ID NO:
- 1, 2, 3, 5, 6, 8, 9, 11, 12, 14, 15, 17, 18, 20, 22, 24, 26, 27, 29, 31, 32, 34, 35, 37, 38, 40, 42, 44, 46, 48, 49, 51 or 52.
Dependencies are inferred from the claim text.