Lepidopteran-Active Cry1Da1 Amino Acid Sequence Variant Proteins

A method of inhibiting Lepidopteran pests from feeding on a crop plant comprising:

• a. modifying one or more amino acid residue(s) of SEQ ID NO:
• 2 or SEQ ID NO:14 through substitution of the one or more amino acid residue(s) to produce a modified SEQ ID NO:2 or SEQ ID NO:14;
• and
• b. making available a Lepidopteran-inhibiting amount of the modified SEQ ID NO:
• 2 or SEQ ID NO:14 within, on the surface, or in the vicinity of tissues of said crop plant;
• wherein the SEQ ID NO:2 or SEQ ID NO:14 modified amino acid residue is selected from the group consisting of serine at position 282 replaced by lysine or valine, tyrosine at position 316 replaced by serine, isoleucine at position 368 replaced by proline or arginine, serine at 374 replaced by arginine, asparagine at position 375 replaced by histidine, and isoleucine at position 432 replaced by leucine.