Method for producing stress tolerant transgenic plant by silencing a gene encoding calcium-dependent lipid-binding protein with c2 domain and applications of the same

Abstract

A method for silencing rice plant, includes the steps of: identifying a first nucleotide sequence from Oryza sativa, wherein the first nucleotide sequence is homologous to a Ca2+-dependent lipid-binding gene of Arabidopsis thaliana (Atclb gene); cloning the first nucleotide sequence to a first vector to form a first recombinant vector, such that the first recombinant vector contains a first insert of the first nucleotide sequence; and transfer the first insert in the first recombinant vector to a second vector to form a second recombinant vector, such that the second recombinant vector contains a second insert of the first nucleotide sequence. The second recombinant vector, when being introduced to Oryza sativa plant cells, is capable of silencing the first nucleotide sequence of the Oryza sativa plant cells, such that the Oryza sativa plant cells are tolerant to an abiotic stress.

Dependencies are inferred from the claim text.