Atsp1, an e3 ubiquitin ligase, and its use
AssigneeUniversity of Leicester
InventorPAUL JARVIS
Life of this patent
Estimating the term…
Abstract
The invention relates to plants with improved phenotypes and related methods. These improved phenotypes are conferred by altering the expression of the SP1 gene which is involved in plastid development or altering the activity of the SP1 protein.
Claims56
A transgenic plant cell, plant or a part thereof characterised in that
- a) the expression of a nucleic acid comprising SEQ ID NO. 1 or 2 and encoding a SP1 peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof is altered or
- b) the activity of a SP1 peptide is altered and said plant expresses a nucleic acid comprising a mutant SEQ ID NO. 1 or 2 nucleic acid encoding a mutant SP1 peptide, a functional homologue or variant thereof which carries a mutation in the RING domain.
A vector comprising a SP1 nucleic acid as defined in SEQ ID NO. 1, 2, 7, 8, 10 or 11, a functional variant or homologue thereof.
A method for altering plastid development in a transgenic plant cell, plant or a part thereof comprising
- a) altering the expression of a nucleic acid comprising SEQ ID NO. 1 or 2 and encoding a SP1 peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof or
- b) altering the activity of a SP1 peptide comprising SEQ ID NO. 3 comprising expressing in a plant a nucleic acid comprising a mutant SEQ ID NO. 1 or 2 and encoding a mutant SP1 peptide, a functional homologue or variant thereof which carries a mutation in the RING domain.
A method for delaying green tissue senescence comprising inactivating, repressing or down-regulating the expression of a nucleic acid comprising SEQ ID NO. 1 and encoding a SP1 peptide comprising SEQ ID NO. 3, a functional homolog or variant thereof.
A method for increasing grain/seed size and/or starch content comprising introducing and expressing in a plant a nucleic acid construct comprising SEQ ID NO. 1 or 2 and encoding a SRI peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof.
A method for increasing yield of a plant comprising increasing, inactivating, repressing or down-regulating the expression of a nucleic acid comprising SEQ ID NO. 1 or 2 and encoding a SP1 peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof or introducing and expressing in a plant a nucleic acid comprising a mutant SEQ ID NO. 1 or 2 and encoding a mutant SP1 peptide, a functional homologue or variant thereof which carries a mutation in the RING domain.
A method for improving seedling emergence, growth and survival comprising introducing and expressing in a plant a nucleic acid construct comprising SEQ ID NO. 1 or 2 and encoding a SRI peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof.
A method for altering fruit ripening in a transgenic plant cell, plant or a part thereof comprising
- a) altering the expression of a nucleic acid comprising SEQ ID NO. 1 or 2 and encoding a SP1 peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof or
- b) altering the activity of a SP1 peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof.
A method for making a transgenic plant with altered plastid development comprising
- a) altering the expression of a nucleic acid comprising SEQ ID NO. 1 or 2 and encoding a SP1 peptide comprising SEQ ID NO, 2, a functional homologue or variant thereof or
- b) altering the activity of a SP1 peptide comprising SEQ ID NO. 2, a functional homologue or variant thereof.
A method for increasing stress tolerance to one or more of salinity, osmotic stress and/or oxidative stress in a plant cell, plant or part thereof comprising introducing and expressing in said plant cell, plant or part thereof a nucleic acid construct comprising a nucleic acid comprising SEQ ID NO. 1 or 2 and encoding a SP1 peptide comprising SEQ ID NO. 3, a functional homologue or variant thereof.
Dependencies are inferred from the claim text.