Back to results
US 2016/0108415 A1Published application
Manipulation of glutamine symthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants
AssigneePioneer Hi-Bred International, Inc.
InventorsRajeev Gupta, Kanwarpal Singh Dhugga
Life of this patent
Estimating the term…
Abstract
The present invention provides polynucleotides and related polypeptides of the protein GS. The invention provides genomic sequence for the GS gene. GS is responsible for controlling nitrogen utilization efficiency in plants. Glutamine synthase sequences are provided for improving grain yield and plant growth. The invention further provides recombinant expression cassettes, host cells and transgenic plants.
Claims26
An isolated polynucleotide selected from the group consisting of:
- a. a polynucleotide having at least 80% sequence identity, as determined by the GAP algorithm under default parameters, to the full length sequence of a polynucleotide selected from the group consisting of SEQ ID NOS:
- 43, 45, 47, 49, 51 and 53;
- wherein the polynucleotide encodes a polypeptide that functions as a modifier of GS;
- b. a polynucleotide encoding a polypeptide selected from the group consisting of SEQ ID NO:
- 44, 46, 48, 50, 52 and 54;
- c. a polynucleotide selected from the group consisting of SEQ ID NOS:
- 43, 45, 47, 49, 51 and 53;
- and
- d. A polynucleotide which is complementary to the polynucleotide of (a), (b) or (c).
6 more elements
A method of decreasing the GS enzyme polypeptide activity in a plant cell, comprising:
- a. providing a nucleotide sequence comprising at least 15 consecutive nucleotides of the complement of SEQ ID NO:
- 43, 45, 47, 49, 51 and 53;
- b. providing a plant cell comprising an mRNA having the sequence set forth in SEQ ID NO:
- 43, 45, 47, 49, 51 and 53;
- and
- c. introducing the nucleotide sequence of step (a) into the plant cell, wherein the nucleotide sequence inhibits expression of the mRNA in the plant cell.
3 more elements
Dependencies are inferred from the claim text.