Methods and compositions for transgenic plants with enhanced cold tolerance, ability to flower without vernalization requirement and impacted fertility
AssigneeClemson University
InventorsHong Luo, Shauangrong Yuan, Zhigang Li, Qian Hu
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Abstract
The present invention provides transgenic plants having increased tolerance to cold and altered flowering characteristics. Also provided are methods and compositions for producing said transgenic plants.
Claims21
A transgenic plant having increased cold tolerance, flowering bypassing vernalization requirement, male sterility and/or altered development, comprising a recombinant nucleic acid molecule, said recombinant nucleic acid molecule comprising a nucleotide sequence encoding miR396c operatively associated with a promoter, wherein overexpression of the nucleotide sequence encoding miR396c confers increased cold tolerance, flowering bypassing vernalization requirement, male sterility and/or altered plant development as compared with a plant that does not comprise said recombinant nucleic acid molecule.
A method of producing a transgenic plant having increased cold tolerance, flowering bypassing vernalization requirement, male sterility and/or altered development, comprising:
- a) transforming a plant cell with a recombinant nucleic acid molecule, said recombinant nucleic acid molecule comprising a nucleotide sequence encoding miR396c and/or other members of the miRNA 396 gene family operatively associated with a promoter;
- and
- b) regenerating a transgenic plant from the transformed plant cell, thereby producing a transgenic plant having increased cold tolerance, flowering bypassing vernalization requirement, male sterility and/or altered development as compared with a plant that is not transformed with said recombinant nucleic acid molecule.
A nucleic acid construct comprising, in the following order from 5′ to 3′:
- a) a first promoter;
- b) a nucleotide sequence encoding miR396c;
- c) a first termination sequence;
- d) a second promoter;
- e) a nucleotide sequence encoding a selectable marker operably associated with the promoter of (d);
- and
- f) a second termination sequence.
Dependencies are inferred from the claim text.