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US 2016/0108393 A1Published application
Rapid Nucleic Acid Extraction Method and Apparatus
OwnerDaniel Laiinventor
Life of this patent
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Abstract
A syringe device having a plunger beneath which is disposed a rupturable capsule containing a nucleic acid wash buffer and a rupturing mechanism for rupturing the capsule, wherein the capsule, syringe, plunger, and rupturing mechanism are so configured as to first apply pneumatic pressure to force a nucleic acid solution through a membrane and to thereafter rupture the capsule and force a first volume of the wash buffer through the membrane.
Claims34
A nucleic acid extraction apparatus comprising:
- a container containing a solution comprising nucleic acid, the container further comprising a membrane configured to capture nucleic acid;
- a rupturable capsule containing a wash buffer;
- a rupturing element for rupturing said capsule so as to release said wash buffer;
- a syringe device, said capsule being positioned within said syringe device, above a lower end of said syringe device and beneath a plunger of said syringe device, the lower end of said syringe device being shaped to fit into an open end of said container;
- the plunger of said syringe device being depressible so as to first force said solution through said membrane such that the membrane retains nucleic acid while passing undesired components of the solution therethrough;
- and
- wherein said plunger is further depressible so as to cause said rupturing element to rupture said capsule and release said wash buffer and to thereafter drive said wash buffer out of the lower end of said syringe device and through said membrane, thereby leaving pure nucleic acid on said membrane.
4 more elements
Nucleic acid extraction apparatus comprising:
- a first container configured to receive a nucleic acid solution ready for extraction and having a membrane located at a lower end thereof, said membrane being configured to capture nucleic acid while passing other components of the solution;
- a capsule containing a buffer solution and having a rupturable seal across a top opening thereof;
- a syringe insertable into an open top end of said first container, the syringe containing a said capsule, said capsule being moveable downwardly within said syringe in response to activation of a plunger of the syringe;
- a spike for rupturing said seal;
- and
- wherein the capsule is so configured and positioned within the syringe that downward movement of the capsule within the syringe first forces the nucleic acid solution in the first container through the membrane, after which the spike ruptures said seal, thereby releasing said buffer solution into said first container.
3 more elements
A nucleic acid extraction apparatus comprising:
- a rupturable capsule containing a wash buffer solution;
- a syringe device containing said capsule, said capsule being disposed beneath a plunger of the syringe device and above a lower open end of the syringe device;
- said capsule having a sealing mechanism which defines first and second chambers within the syringe device such that movement of said plunger downwardly causes downward movement of said capsule within said plunger and forces a fluid out of said open end.
A nucleic acid extraction apparatus comprising:
- a capsule containing a wash buffer solution and having a top surface comprising a rupturable seal;
- a syringe device containing said capsule, said capsule being disposed beneath a plunger of the syringe device and above a lower open end of the syringe device;
- said capsule having a sealing mechanism which defines first and second chambers within the syringe device such that movement of said plunger downwardly causes downward movement of said capsule within said syringe device;
- and
- a piercing element for piercing said seal located at a lower end of said plunger.
2 more elements
Dependencies are inferred from the claim text.