Hydrolases, nucleic acids encoding them and methods for making and using them
AssigneeDsm IP Assets B.V.
InventorsTim HITCHMAN, Christopher L.G. DAYTON, Katie A. KLINE, Jonathan LYON, Mark A. WALL, Nelson R. BARTON
Life of this patent
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Abstract
Provided are hydrolases, including lipases, saturases, palmitases and/or stearatases, and polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. Further provided are polypeptides, e.g., enzymes, having a hydrolase activity, e.g., lipases, saturases, palmitases and/or stearatases and methods for preparing low saturate or low trans fat oils, such as low saturate or low trans fat animal or vegetable oils, e.g., soy or canola oils.
Claims17
An isolated, synthetic or recombinant polynucleotide comprising a nucleic acid sequence that encodes the polypeptide of claim 3.
An isolated, synthetic or recombinant variant polypeptide comprising an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO:2, and at least one amino acid residue substitution to the amino acid sequence of SEQ ID NO:2, wherein the amino acid residue substitution is selected from the group consisting of:
- Y7R, D16M, G27Q, G27S, L43V, G45A, G45L, A48T, S54H, D61A, D61E, D61S, V62E, V62A, V62G, V62M, V62N, V62Q, V62S, V62T, R72E, R72K, R72P, R72S, R72T, R72Y, F741, F74L, F74P, F74R, G77P, G80P, V83C, V83M, D84V, R89S, A96C, A961, A96S, G98A, G98L, Y113F, E116A, E116F, E116G, E116H, E116L, E116P, E116Q, E116R, E1165, E116T, E116V, K1201, K120L, K120F, K120M, K1205, K146A, I147F, I147L, I151A, I151G, I151H, I151P, I151S, I151T, T155C, D157S, D157G, D268T, N158A, L159M, P160T, I167R, I167S, R172P, R172Q, R172S, D197K, A210V, A211T, A211S, A211I, S212C, S212A, S212E, S212G, S212H, S212L, S212P, S212Q, S212R, S212T, S212V, S212W, S212Y, K213I, K213G, K213T, T214V, T214P, T214N, T214R, T214Y, G215A, G215H, G215S, G215M, G215V, G215P, G215C, G215W, A216T, A216R, A216Y, A216V, A216C, A216C, A216S, A216L, E217Q, E217R, E217S, E217A, E217G, E217P, A218M, A218H, A218Q, A218R, A218W, A218W, A218S, A218T, A218T, A218K, V223A, V223M, V223R, V223T, V223T, V223F, A224F, A224G, A224I, A224Q, A224Y, A225G, A225L, A225M, A225Q, and A225T, and the variant polypeptide has palmitase activity.
An isolated, synthetic, or recombinant variant polypeptide comprising the amino acid sequence selected from the group consisting of:
- (a) a variant polypeptide comprising an amino acid sequence that is at least 85% identical to the amino acid sequence as set forth in SEQ ID NO:2 and the combination of amino acid substitutions of FIG. 8;
- (b) a variant polypeptide comprising an amino acid sequence that is at least 85% identical to the amino acid sequence as set forth in SEQ ID NO:2 and the combination of amino acid substitutions of Table 5;
- (c) a variant polypeptide comprising an amino acid sequence that is at least 85% identical to the amino acid sequence as set forth in SEQ ID NO:2 and the combination of amino acid substitutions of Table 6;
- wherein the variant polypeptide has palmitase activity.
Dependencies are inferred from the claim text.