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AssigneeLife Technologies Corporation
InventorsJun LEE, Gary Gerard, Harini Shandilya, Katherine Griffiths, Moreland Gibbs, Peter Bergquist, Robert Potter
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The present invention provides polypeptides having a nucleotide polymerase activity and method of enhancing polymerase activity. The polypeptides of the present invention may possess both a DNA-dependent DNA polymerase activity and an RNA-dependent DNA polymerase activity, i.e., a reverse transcriptase activity. The polypeptides of the present invention may be used in any application including, but not limited to, DNA sequencing reactions, amplification reactions, cDNA synthesis reactions, and combined cDNA synthesis and amplification reactions, e.g., RT-PCR.
1-122. (canceled)
An isolated nucleic acid comprising a nucleotide sequence encoding a polypeptide comprising an amino acid sequence that is at least 80% identical to at least forty contiguous amino acids in any one of SEQ ID NOS:
A polypeptide comprising an amino acid sequence that is at least 80% identical to at least forty contiguous amino acids in any one of SEQ ID NOS:
A method of amplifying a double-stranded DNA molecule comprising:
Dependencies are inferred from the claim text.