Mdck-derived cell strain suspension-cultured in protein-free medium and method for proliferating virus using cell strain
AssigneeMogam Biotechnology Institute
InventorsMihee HWANG, Kukjin PARK, Duckhyang SHIN, Hyeon LEE, Sooin KIM, Eunyoung CHO, Misuk KIM, Dong Ho AHN
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Abstract
The present invention relates to a novel MDCK-derived cell line capable of being suspension-cultured in a protein-free medium and a method for proliferating a virus using the MDCK-derived cell line to produce a vaccine. The novel MDCK-derived cell line exhibits high and uniform productivity for various viruses, while causing less viral antigenic variations with low tumorigenicity, and thus can be useful in producing viruses used for vaccines.
Claims6
A Madin-Darby canine kidney (MDCK)-derived MDCKS-MG cell line capable of being suspension-cultured in a protein-free medium (Accession No. KCLRF-BP-00297).
A method for proliferating a virus to produce a vaccine, which comprises the steps of:
- 1) infecting a MDCK-derived MDCKS-MG cell line (Accession No. KCLRF-BP-00297) with the virus;
- 2) culturing the MDCK-derived MDCKS-MG cell line infected with the virus;
- and
- 3) isolating the virus from the cell culture obtained in step 2).
Dependencies are inferred from the claim text.