Culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells
AssigneeNational Chung Hsing University
InventorsHong-Lin Su, Sheng-Mei Chen
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Abstract
The present invention discloses a culture medium and method for inducing differentiation of pluripotent stem cells into neuroepithelial cells. The culture medium comprises at least two agents for neural induction, wherein the agents includes a Wnt signal agonist and a Smad2/3 inhibitor and the culture medium is a feeder free culture. The method is to culture pluripotent stem cells in the culture medium to differentiate into neuroepithelial cells. The neuroepithelial cells can further differentiate into mature neurons for practical applications, including regeneration medicine and drug discovery for neural disorders.
Claims21
A culture medium for inducing differentiation of pluripotent stem cells into neuroepithelial cells comprising at least two agents for neural induction, wherein the agents include a Wnt signal agonist and a Smad2/3 inhibitor and the culture medium is a feeder free culture.
An additive to a culture medium for triggering pluripotent stem cells into neuroepithelial cells includes a Wnt signal agonist and a Smad2/3 inhibitor.
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