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US 2016/0108361 A1Published application
Media compositions for neuronal cell culture
AssigneeThe Salk Institute for Biological Studies
InventorsFred H. Gage, Cedric Bardy
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Abstract
Provided herein are, inter alia, are media compositions useful for culturing neural cells. In particular, the compositions provided herein mimic important physiological conditions in the living brain and sustain neural activity. The media compositions provided herein improve the efficiency of human neuron maturation and promote synaptic function in long-term in vitro cultures.
Claims24
A cell medium comprising:
- (a) Sodium chloride at a concentration of between about 70 and about 150 mM;
- (b) a neuroactive inorganic salt at a concentration of between about 0.000001 and about 10 mM;
- (c) Glycine at a concentration of between about 0.0001 and about 0.05 mM;
- (d) L-alanine at a concentration of between about 00001 and about 0.05 mM;
- and
- (e) L-serine at a concentration of between about 0.001 and about 0.03 mM.
3 more elements
A method of culturing a neuronal cell comprising contacting a neuronal cell with a medium comprising
- (a) Sodium chloride at a concentration of between about 70 and about 150 mM;
- (b) a neuroactive inorganic salt at a concentration of between about 0.000001 and about 10 mM;
- (c) Glycine at a concentration of between about 0.0001 and about 0.05 mM;
- (d) L-alanine at a concentration of between about 00001 and about 0.05 mM;
- and
- (e) L-serine at a concentration of between about 0.001 and about 0.03 mM.
3 more elements
A mammalian cell cultured in a medium comprising
- (a) Sodium chloride at a concentration of between about 70 and about 150 mM;
- (b) a neuroactive inorganic salt at a concentration of between about 0.000001 and about 10 mM;
- (c) Glycine at a concentration of between about 0.0001 and about 0.05 mM;
- (d) L-alanine at a concentration of between about 00001 and about 0.05 mM;
- and
- (e) L-serine at a concentration of between about 0.001 and about 0.03 mM.
3 more elements
Dependencies are inferred from the claim text.