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US 2016/0108357 A1Published application
Methods of culturing a cell
AssigneeAlexion Pharmaceuticals, Inc.
InventorsHunter F. Malanson, Pratik Jaluria
Life of this patent
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Abstract
Provided herein are methods of culturing a mammalian cell that include culturing a mammalian cell comprising a recombinant protein-encoding nucleic acid in a liquid culture medium containing 0.1 g/L or more processed Bovine Serum Albumin (BSA) under conditions sufficient to produce the recombinant protein, where the processed BSA is present in and/or added to the liquid culture medium prior to and/or during the culturing step.
Claims19
A method of culturing a mammalian cell, the method comprising:
- fed batch culturing a NS0 cell comprising a recombinant eculizumab-encoding nucleic acid in a liquid culture medium comprising 0.1 g/L or more processed Bovine Serum Albumin (BSA) under conditions sufficient to produce the recombinant eculizumab, wherein the processed BSA is present in and/or added to the liquid culture medium prior to and/or during the culturing step.
Dependencies are inferred from the claim text.