Recombinant antibody composition

A process for producing recombinant antibodies, comprising culturing a transformant in a medium to form and accumulate the recombinant antibodies in the culture;

• and recovering and purifying the recombinant antibodies from the culture,
• wherein said recombinant antibodies have greater complement-dependent cytotoxic activity than human IgG1 and human IgG3 antibodies of the same binding specificity under the same conditions,
• wherein said recombinant antibodies contain a heavy chain constant region containing, a CHI, a hinge, a CH2 and a CH3 domain, said region comprising the amino acid sequence of SEQ ID NO:76 in which at least the first 110 amino acids commencing at residue 114 of SEQ ID NO:76 are replaced with the corresponding residues from SEQ ID NO:78, and in which residues 1-113 of SEQ ID NO:76 in said region are not replaced with the corresponding residues from SEQ ID NO:78,
• and wherein said transformant contains DNA encoding said recombinant antibodies.

A process for producing recombinant antibodies, comprising culturing a transformant in a medium to form and accumulate the recombinant antibodies in the culture;

• and recovering and purifying the recombinant antibodies from, the culture,
• wherein said recombinant antibodies have greater complement-dependent cytotoxic activity than human IgG1 and human IgG3 antibodies of the same binding specificity under the same conditions,
• wherein said recombinant antibodies contain a heavy chain cons I:ant region containing CHI, a binge, a CH2 and a CH3 domain, said region comprising the amino acid sequence of SEQ ID NO:76 in which residues selected from the following (1)-(8) are replaced, with the corresponding residues from SEQ ID NO:78:
• (1) residues 114-223 of SEQ ID NO:76
• (2) residues 114-239 of SEQ ID NO:76;
• (3) residues 114-241 of SEQ ID NO:76;
• (4) residues 114-267 of SEQ ID No:76;
• (5) residues 114-275 of SEQ ID NO:76;
• (6) residues 114-280 of SEQ 1D NO:76;
• (7) residues 114-305 of SEQ ID NO:76;
• and
• (8) residues 114-317 and residues 319-330 of SEQ ID NO:76,
• and wherein said transformant contains DNA encoding said recombinant antibodies.