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US 2016/0108119 A1Published application
Protein purification
AssigneeUCB Pharma S.A.
InventorsMariangela Spitali, Jonathan Symmons, Richard Whitcombe, Mark Robert Pearce-Higgins
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Abstract
The present invention relates to a process for the purification of an antibody fragment from a periplasmic cell extract comprising a first cation exchange chromatography step and a second anion exchange chromatography step.
Claims20
A process for the purification of an antibody fragment comprising:
- a) a first chromatography step to capture the antibody fragment wherein a periplasmic cell extract containing an antibody fragment at a concentration of at least 1.5 g/L is subjected to cation exchange chromatography and subsequently eluted to produce a first eluate containing the antibody fragment;
- and
- b) a second chromatography step wherein the first eluate is subjected to anion exchange chromatography to capture impurities and produce a flow through containing the antibody fragment.
A process for the purification of an antibody fragment from periplasmic cell extract consisting essentially of:
- a) a first chromatography step to capture the antibody fragment wherein a mixture containing an antibody fragment at a concentration of at least 1.5 g/L is subjected to cation exchange chromatography and subsequently eluted to produce a first eluate containing the antibody fragment;
- b) a first ultrafiltration applied to the first eluate;
- c) a second chromatography step wherein the purified first eluate is subjected to anion exchange chromatography to capture impurities and produce a flow through containing the antibody fragment;
- and
- d) a second ultrafiltration applied to the flow through.
2 more elements
Dependencies are inferred from the claim text.