Anti-TNF-alpha/CXCL10 Double-Targeting Antibody and Use Thereof

A TNF-α/CXCL-10 double-targeting antibody, comprising:

• a first antigen-binding site specifically binding to tumor necrosis factor-alpha (TNF-α);
• and
• a second antigen-binding site specifically binding to C-X-C motif chemokine 10 (CXCL10),
• wherein
• the first antigen-binding site comprises a heavy chain variable domain (VH) including a heavy chain complementarity determining region (HCDR) 1 having the amino acids of SEQ ID NO:
• 1, an HCDR2 having the amino acids of SEQ ID NO:
• 2, and an HCDR3 having the amino acids of SEQ ID NO:
• 3, and a light chain variable domain (VL) including a light chain complementarity determining region (LCDR) 1 having the amino acids of SEQ ID NO:
• 5, an LCDR2 having the amino acids of SEQ ID NO:
• 6, and an LCDR3 having the amino acids of SEQ ID NO:
• 7, and
• the second antigen-binding site comprises a heavy chain variable domain (VH) including the HCDR1 having the amino acids selected from the group consisting of SEQ ID NOs:
• 9, 17, 21 and 25, the HCDR2 having the amino acids selected from the group consisting of SEQ ID NOs:
• 10, 18, 22 and 26, and the HCDR3 having the amino acids selected from the group consisting of SEQ ID NOs:
• 11, 19, 23 and 27, and a light chain variable domain (VL) including the LCDR1 having the amino acids selected from the group consisting of SEQ ID NOs:
• 13, 29, 33 and 37, the LCDR2 having the amino acids selected from the group consisting of SEQ ID NOs:
• 14, 30, 34 and 38, and the LCDR3 having the amino acids selected from the group consisting of SEQ ID NOs:
• 15, 31, 35 and 39.