Immunogenic peptides and their use in transplantation
AssigneeImcyse SA
InventorJean-Marie Saint-Remy
Life of this patent
Estimating the term…
Abstract
The present invention relates to the use of immunogenic peptides comprising a T-cell epitope derived from an allograft antigen and a redox motif such as C-(X)2-[CST] or [CST]-(X)2-C in the prevention and/or treatment of allograft rejection and in the manufacture of medicaments therefore.
Claims22
An isolated immunogenic peptide with a length of between 12 and 75 amino acids comprising:
- (i) an MHC class II T-cell epitope of an alloantigenic protein of an allograft, and
- (ii) C-(X)2-[CST] or [CST]-(X)2-C redox motif,
- wherein said epitope and said motif are immediately adjacent to each other or are separated by at most 7 amino acids.
A method for preparing an immunogenic peptide capable of eliciting a population of allograft antigen-specific cytolytic CD4+ T cells, the method comprising the steps of:
- a) identifying in an alloantigenic protein of an allograft an MHC class II T cell epitope, and
- b) synthesising a peptide of between 12 and 75 amino acids comprising the se sequence of said identified MHC class II T cell epitope and comprising an C-(X)2-[CST] or [CST]-(X)2-C redox motif sequence whereby said epitope and said motif are immediately adjacent to each other or are separated by at most 7 amino acids.
A method for obtaining a population of allograft antigen-specific cytolytic CD4+ T cells, the method comprising the steps of:
- providing peripheral blood cells;
- contacting said cells with an immunogenic peptide of between 12 and 75 amino acids comprising (i) an MCH class II T-cell epitope of an allograft antigenic protein and (ii) a C-(X)2-[CST] or [CST]-(X)2-C redox motif wherein said epitope and said motif are immediately adjacent to each other or are separated by at most 7 amino acids;
- and
- expanding said cells in the presence of IL-2.
A method for obtaining a population of allograft antigen-specific cytolytic CD4+ T cells, the method comprising the steps of:
- providing an immunogenic peptide of between 12 and 75 amino acids comprising
- (i) an MHC class II T-cell epitope of an allograft antigenic protein and
- (ii) a C-(X)2-[CST] or [CST]-(X)2-C redox motif wherein said epitope and said motif are immediately adjacent to each other or are separated by at most 7 amino acids;
- administering said immunogenic peptide to a subject;
- and
- obtaining said population of allograft antigen-specific cytolytic CD4+ T cells from said subject.
A method for preventing or treating in a mammalian recipient the rejection of an allograft comprising the steps of administering to said recipient, prior to or after introducing said allograft, at least one isolated immunogenic peptide with a length of between 12 and 75 amino acids comprising:
- (a) an MHC class II T-cell epitope of an alloantigenic protein of said allograft and
- (b) a C-(X)2-[CST] or [CST]-(X)2-C redox motif, wherein said epitope and said motif are immediately adjacent to each other or are separated by at most 7 amino acids.
Dependencies are inferred from the claim text.