Synthesis Of 2',3'-Dideoxynucleosides For Automated DNA Synthesis And Pyrophosphorolysis Activated Polymerization
AssigneeLife Technologies Corporation
InventorsZhaochun MA, Khairuzzaman Bashar MULLAH, Robert G. EASON
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Abstract
Methods for preparation of 2′,3′-dideoxynucleotides support structures, such as 2′,3′-dideoxyguanosine, 2′,3′-dideoxyadenosine, and 3′-deoxythymidine support structures are disclosed. Various methods of using such structures are also provided, such as their use for automated DNA synthesis and pyrophosphorolysis activated polymerization.
Claims18
-4. (canceled)
A method for preparing a 2′,3′-dideoxyadenosine support structure, the method comprising:
- (a) reacting 2′,3′-dideoxyadenosine with 4,4-dimethoxytriphenylmethyl chloride to obtain a first intermediate;
- (b) reacting pentane-1,3,5-tricarboxylic acid with oxalyl chloride to obtain a second intermediate;
- (c) reacting the first intermediate with the second intermediate to obtain a 2′,3′-dideoxyadenosine derivative;
- and
- (d) coupling the 2′,3′-dideoxyadenosine derivative to a solid support, thereby obtaining the 2′,3′-dideoxyadenosine support structure.
(canceled)
-27. (canceled)
The method of claim 35, wherein the phosphosdiester linkages are selected from the group consisting of phosphodiester, phosphorothioate, phosphorodithioate, phosphoroselenoate, phosphorodiselenoate, phosphoroanilothioate, phosphoranilidate, and phosphoramidite.
Dependencies are inferred from the claim text.