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US 2016/0107951 A1Published application
Digital microfluidic platform for radiochemistry
AssigneeThe Regents of the University of California
InventorsR. Michael Van Dam, Chang-Jin Kim, Supin Chen, Huijiang Ding, Gaurav Jitendra Shah, Pei Yuin Keng
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Abstract
Disclosed herein are methods of performing microchemical reactions and electro-wetting-on-dielectric devices (EWOD devices) for use in performing those reactions. These devices and method are particularly suited for preparing radiochemical compounds, particularly compounds containing 18F.
Claims17
1-40. (canceled)
A method of performing radiosynthesis comprising:
- providing an electro-wetting-on-dielectric device (EWOD) comprising:
- a first substrate having one or more electrically defined fluid paths along a first surface thereof, each fluid path comprising discrete electrode pads, wherein adjacent electrode pads are separated from each other by non-conductive spaces, each of the electrode pads having electrically conductive lines attached thereto, each conductive line provided to deliver electrical signals to the conductive pad to which the line is attached to provide electrically directed movement of one or more fluid droplets along the fluid paths;
- one or more fluid delivery sites located on or adjacent to the one or more electrically defined fluid paths;
- one or more process operation sites located along the electrically defined fluid paths on the first substrate;
- delivering one or more fluid droplets comprising a radioisotope in a liquid carrier to one of the process operation sites;
- delivering one or more fluid droplets comprising at least one radiosynthesis reagent to one of the fluid delivery sites;
- providing an electric field in a serial manner to the conductive pads adjacent to the fluid delivery sites to move the one or more fluid droplets comprising the at least one radiosynthesis reagent to process operation site with the radioisotope;
- reacting at least one of the radiosynthesis reagents with the radioisotope at the process operation site;
- and
- recovering the reaction product from the EWOD device.
7 more elements
A method of performing Fluorine-18 radiosynthesis comprising:
- providing an electro-wetting-on-dielectric device (EWOD) comprising:
- a first substrate having one or more electrically defined fluid paths along a first surface thereof, each fluid path comprising discrete electrode pads, wherein adjacent electrode pads are separated from each other by non-conductive spaces, each of the electrode pads having electrically conductive lines attached thereto, each conductive line provided to deliver electrical signals to the conductive pad to which the line is attached to provide electrically directed movement of one or more fluid droplets along the fluid paths;
- one or more fluid delivery sites located on or adjacent the one or more electrically defined fluid paths;
- one or more process operation sites located along the electrically defined fluid paths on the first substrate;
- delivering one or more fluid droplets comprising [18F]fluoride in a liquid carrier to one or more of the process operation sites;
- evaporating the one or more fluid droplets comprising [18F]fluoride at the one or more process operation sites;
- delivering one or more fluid droplets comprising a precursor containing in an organic liquid carrier at one of the fluid delivery sites;
- providing an electric field in a serial manner to the conductive pads adjacent to the fluid delivery sites to move the one or more fluid droplets comprising the precursor to the one or more process operation sites containing the evaporated [18F]fluoride;
- heating the one or more process operation sites containing the evaporated [18F]fluoride and the precursor to generate a reaction product;
- and
- recovering the reaction product from the EWOD device.
8 more elements
The method of claim 40, wherein evaporating the one or more fluid droplets comprising [18F]fluoride at the one or more one or more process operation sites comprises adding a plurality of droplets of an anhydrous organic solvent while the one or more heater sites are actively heating the one or more fluid droplets comprising [18F]fluoride.
A method of performing radiosynthesis comprising:
- providing an electro-wetting-on-dielectric device (EWOD) comprising:
- a first substrate having one or more electrically defined fluid paths along a first surface thereof, each fluid path comprising discrete electrode pads, wherein adjacent electrode pads are separated from each other by non-conductive spaces, each of the electrode pads having electrically conductive lines attached thereto, each conductive line provided to deliver electrical signals to the conductive pad to which the line is attached to provide electrically directed movement of one or more fluid droplets along the fluid paths;
- one or more fluid delivery sites located on or adjacent to the one or more electrically defined fluid paths;
- one or more process operation sites located along the electrically defined fluid paths on the first substrate;
- delivering one or more fluid droplets comprising at least one radiosynthesis reagent to one of the process operation sites;
- delivering one or more fluid droplets comprising a radioisotope in a liquid carrier to one of the fluid delivery sites, the delivery site being spaced from the one or more operation sites;
- providing an electric field in a serial manner to the conductive pads adjacent to the fluid delivery sites to move the one or more fluid droplets comprising the radioisotope to the process operation site containing the at least one radiosynthesis reagent;
- reacting at least one of the radiosynthesis reagents with the radioisotope at the process operation site;
- and
- recovering the reaction product from the EWOD device.
7 more elements
Dependencies are inferred from the claim text.