Adaptation of attenuated infectious bronchitis virus (ibv) to embryonic kidney cells and vaccine thereby produced
AssigneeAuburn University
InventorHaroldo E. Toro
Life of this patent
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Abstract
Disclosed are methods for preparing a vaccine against infection by infectious bronchitis virus (IBV). The methods typically include passing a heterogeneous attenuated population of IBV in chicken embryonic kidney cells, and optionally may include further passaging the heterogeneous attenuated population of IBV in embryonated chicken eggs (ECE) in order to obtain passaged attenuated population of IBV. Also disclosed are passaged attenuated populations of IBV in which the populations display a desired degree of homogeneity. Also disclosed are vaccines comprising the passaged attenuated populations of IBV and methods of vaccination comprising administering the disclosed vaccines.
Claims20
A method for preparing a vaccine against infection by infectious bronchitis virus (IBV), the method comprising passing a heterogeneous attenuated population of IBV in chicken embryonic kidney cells.
A vaccine comprising a passaged attenuated population of IBV and a suitable carrier or excipient, wherein at least about 95% of the passaged attenuated population of IBV exhibits homogeneity at one or more amino acid positions in the S1 polypeptide selected from the group consisting of Ser at amino acid position 213 of the S1 polypeptide, Arg at amino acid position 323 of the S1 polypeptide, Arg at amino acid position 386 of the S1 polypeptide, Gln at amino acid position 398 of the S1 polypeptide, His at amino acid position 399 of the S1 polypeptide, and any combination thereof.
Dependencies are inferred from the claim text.