Neutrophil-depleted whole blood and platelet rich plasma compositions

A cell separation device for producing neutrophil-depleted platelet-rich plasma with centrifugation, said device comprising:

• an inlet portion comprising an inlet port for receiving a sample of whole blood;
• a separation chamber adapted to separate platelet-rich plasma from other components of the whole blood based on differences in specific gravity;
• a flow path for the platelet-rich plasma comprising an area of constriction adapted to remove neutrophils from the platelet rich plasma;
• and
• an outlet portion comprising a collection port in fluid communication with the separation chamber for removing neutrophil-depleted platelet-rich plasma.

A kit for treating damaged tissue comprising:

• (i) a syringe for withdrawing blood from a patient;
• (ii) a centrifuge device to obtain neutrophil-depleted platelet-rich plasma;
• the centrifuge device comprising:
• an inlet portion comprising an inlet port for receiving a sample of blood;
• a separation chamber adapted to separate platelet-rich plasma from other components of the blood based on differences in specific gravity;
• a flow path for the platelet-rich plasma comprising an area of constriction adapted to remove neutrophils from the platelet rich plasma;
• and
• an outlet portion comprising a collection port in fluid communication with the separation chamber for removing neutrophil-depleted platelet-rich plasma;
• and
• (iii) a syringe for subcutaneous delivery of the neutrophil-depleted platelet-rich plasma to the damaged tissue.