Freeze preservation of living cells
AssigneeUniversité De Namur
InventorsChristophe MEUNIER, Pierre VAN CUTSEM, Bao-Lian SU
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Abstract
A method is for the cryopreservation of a (plant) cell, (plant) organelle or (plant) tissue, where the (plant) cell, (plant) organelle or (plant) tissue is encapsulated in a matrix formed of an abiotic material in presence of one or more additives with cryoprotectant effect. The method provides a faster, more effective and cheaper way to cryopreserve biological material.
Claims17
A method for cryopreservation of a plant cell, a plant cell organelle or plant tissue, which comprises the steps of:
- encapsulating said plant cell, plant cell organelle or plant tissue within a porous silica matrix comprising one or more silica precursor(s) and one or more cryoprotectant(s);
- incubating an obtained mixture at a temperature between 4° C. and 20° C. for a period of more than 1 hour;
- and
- transferring resulting hybrid silica gels in a freezer at a temperature between −30° C. and −196° C.
A cryopreservation kit for a plant cell, a plant cell organelle or a plant tissue comprising one or more silica precursors and one or more cryoprotectant(s).
Dependencies are inferred from the claim text.