Improved hybrid seed production method
AssigneeBayer CropScience AG
InventorsFrank Michiels, KIrk Johnson
Life of this patent
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Abstract
Methods are provided for hybrid seed production using 3-phyletic crosses between female, maintainer and male, particularly restorer lines, wherein the trait is introduced in the female line only at the stage of basic seed production via crossing of the female line with a maintainer line containing the gene or genes encoding the trait in homozygous state.
Claims33
1) A method for producing herbicide-tolerant hybrid seed using
- a) a male-sterile plant line or A-line;
- b) a male-sterile plant line comprising a herbicide tolerance gene in heterozygous or hemizygous state or AHT-line;
- c) an isogenic maintainer plant line or B-line;
- d) an isogenic maintainer plant line comprising a herbicide tolerance gene in homozygous state or BHT-line;
- e) and a male fertile line which can be a restorer line or R-line comprising said herbicide tolerance gene in homozygous state;
- wherein said hybrid seed is produced by crossing plants grown from basic seed of the AHT-line and plants of the male fertile line or R-line and collecting the seeds produced on plants of the A-line, and
- wherein said basic seed of the AHT-line has been produced by crossing plants grown from pre-basic seed of the A-line with plants of the BHT-line and collecting the seeds produced on plants of the A-line, and
- wherein said pre-basic seed of the A-line has been produced by crossing plants grown from pre-basic seed of the A-line with plants of the B-line and collecting seeds produced on plants of the A-line.
2) A method for producing herbicide-tolerant hybrid seed using
- a) a male-sterile plant line (A-line);
- b) a male-sterile plant line comprising a herbicide tolerance gene in heterozygous or hemizygous state (AHT-line);
- c) an isogenic maintainer plant line (B-line);
- d) an isogenic maintainer plant line comprising a herbicide tolerance gene in homozygous state (BHT-line);
- e) and a male fertile line which may be a restorer line comprising said herbicide tolerance gene in homozygous state (R-line);
- said method comprising the steps of
- i. producing pre-basic seed of the A-line by crossing plants grown from pre-basic seed of the A-line with plants of the B-line and collecting seeds produced on plants of the A-line;
- ii. producing basic seed of the AHT-line by crossing plants grown from said pre-basic seed of the A-line with plants of the BHT-line and collecting the seeds produced on plants of the A-line;
- and
- iii. producing hybrid seed by crossing plants grown from basic seed of the AHT-line and plants of the male fertile line or R-line and collecting the seeds produced on plants of the AHT-line.
3) In a method for producing herbicide tolerant hybrid seeds using a three-line hybridization system comprising a male-sterile plant line or A-line, an isogenic maintainer plant line or B-line and a male fertile line, particularly a restorer liner or R-line comprising a herbicide tolerance gene, the improvement of crossing a fourth isogenic maintainer line further comprising said herbicide tolerance gene in homozygous state with said male-sterile plant only at the stage of basic seed production and not at any pre-basic seed production stages.
32) A method for producing hybrid seed comprising a trait of interest using
- a) a male-sterile plant line or A-line;
- b) a male-sterile plant line comprising said trait of interest in heterozygous or hemizygous state or AGOI-line;
- c) an isogenic maintainer plant line or B-line;
- d) an isogenic maintainer plant line comprising said trait of interest in homozygous state or BGOI-line;
- e) and a male fertile line, particularly a restorer line or R-line comprising said trait of interest in homozygous state;
- wherein said hybrid seed is produced by crossing of basic seed of the AGOI-line and the male fertile line or R-line and collecting the seeds produced on plants of the A-line, and
- wherein said basic seed of the AGOI-line has been produced by crossing pre-basic seed of the A-line with pre-basic seed of the BGOI-line and collecting the seeds produced on plants of the AGOI-line, and
- wherein said pre-basic seed of the A-line has been produced by crossing pre-basic seed of the A-line with pre-basic seed of the B-line and collecting seeds produced on plants of the A-line.
Dependencies are inferred from the claim text.