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US 2016/0103141 A1Published application
Compositions and methods for detecting vitamin d
AssigneeSiemens Healthcare Diagnostics Inc.
InventorsZhu Teng, Roland Janzen, Martin A. Drinan
Life of this patent
Estimating the term…
Abstract
Compounds include carbamate derivatives of vitamin D including vitamin D3 and vitamin D2. The compounds are useful in methods and kits for determining the presence and/or amount of vitamin D including vitamin D analogs and metabolites thereof in a sample suspected of containing the same.
Claims20
A compound of the formula:
- wherein:
- Z is an alkyl, alkenyl or alkynyl group having 1 to 10 carbon atoms, which may be unsubstituted or one or more of which may be substituted by one or more of hydroxy, lower alkoxy, oxo, and oxime;
- —NH—O-(A)k-R2 wherein R2 is a member of a signal producing system;
- k is 0 or 1;
- A is a bond when k is 0 and a linking group when k is 1;
- and
- R4 is H, OH or lower alkoxy.
4 more elements
A compound of the formula:
- or a compound of the formula:
- wherein:
- —NH—O-(A)k-R2, wherein R2 is a member of a signal producing system;
- k is 0 or 1;
- A is a bond when k is 0 and a linking group when k is 1;
- R1 is H, OH, or protected OH;
- and
- R4 is H, OH or lower alkoxy.
5 more elements
A compound of the formula:
- or a compound of the formula:
- wherein:
- L is selected from the group consisting of magnetic particles, acridinium esters, a combination of magnetic particles and acridinium esters, chemiluminescent particles and sensitizer particles;
- and R1′ is OH and A′ is a bond or a linking group.
1 more element
A method of determining in a sample the presence and/or amount of vitamin D, the method comprising:
- (a) providing in combination in a medium:
- (i) the sample,
- (ii) a compound of the formula:
- or a compound of the formula:
- wherein:
- R″ is —NH—O-A″-R2′, wherein R2′ is a member of a signal producing system and A″ is a bond or a linking group, and
- R1″ is H or OH;
- and
- (iii) a specific binding member for vitamin D;
- (b) subjecting the combination to conditions for binding of the compound of to the specific binding member to form a complex, and
- (c) measuring the amount of the complex, the amount of the complex being related to the presence and/or amount of vitamin D in the sample.
8 more elements
Dependencies are inferred from the claim text.