Method
AssigneesOxford Radcliffe Hospital Nhs ("Orh") Trust of the John Radcliffe Hospital, King's College London, Guy's & St. Thomas' Hospital Nhs Trust ("Gst")
InventorsMartin BARNARDO, Andrea Harmer, Michael Bunce, Robert Vaughan, Kenneth Welsh
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Abstract
The invention provides a method of detecting the presence of anti-MHC antibodies in a sample comprising contacting said sample with one or more recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof and detecting the binding or absence of binding of antibodies to said recombinant MHC molecules. This method allows the detection and/or identification of one or more specific MHC particularly HLA allele antibodies.
Claims21
A method of detecting the presence of anti-MHC antibodies in a sample comprising contacting said sample with one or more recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof and detecting the binding or absence of binding of antibodies to said recombinant MHC molecules, variants, derivatives or fragments thereof.
A method of detecting the presence of anti-HLA antibodies in a sample comprising contacting said sample with one or more recombinant HLA molecules or functionally equivalent variants, derivatives or fragments thereof and detecting the binding or absence of binding of antibodies to said recombinant HLA molecules, variants, derivatives or fragments thereof.
A single solid support carrying more than 2 different recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof at discrete locations on said solid support.
A kit comprising at least the following components:
- a) one or more recombinant MHC molecules or functionally equivalent variants, derivatives or fragments thereof;
- b) optionally a solid support, together with means for attachment of the MHC molecules;
- and
- c) a means for detecting anti-MHC-antibodies, preferably an antibody which binds to the complex formed between said MHC molecules and naturally occurring antibodies to said molecules.
Dependencies are inferred from the claim text.