Methods for detecting a mycobacterium tuberculosis infection
AssigneesOregon Health & Science University, The United States Government as represented by the Department of Veterans Affairs
InventorsDavid M. Lewinsohn, Deborah A. Lewinsohn
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Abstract
Methods for detecting an infection with Mtb in a subject are disclosed. The methods include detecting the presence of CD8+ T cells that specifically recognize an Mtb polypeptide. The methods include in vitro assays for detecting the presence of CD8+ T cells in a biological sample, and in vivo assays that detect a delayed type hypersensitivity reaction. The methods also include detecting Mtb polypeptides and polynucleotides.
Claims23
A method for detecting Mycobacterium tuberculosis in a subject, comprising
- contacting a biological sample from the subject comprising T cells ex vivo with one or more Mycobacterium polypeptides, and an antigen presenting cell presenting the one or more Mycobacterium polypeptides wherein the one or more Mycobacterium polypeptides
- (a) the amino acid sequence set forth as SEQ ID NO:
- 6;
- or
- (b) at least nine to twenty consecutive amino acids of at least one of the amino acid sequence set forth as SEQ ID NO:
- 6, wherein the nine to twenty consecutive amino acids specifically bind major histocompatibility complex (MHC) class I;
- and
- determining if the T cells specifically recognize the Mycobacterium polypeptide, wherein the presence of T cells that specifically recognize the Mycobacterium polypeptide detects Mycobacterium tuberculosis in the subject.
(canceled)
A method of detecting a Mycobacterium tuberculosis infection in a subject, comprising
- detecting the presence of a Mycobacterium polypeptide or a polynucleotide encoding the polypeptide in a sample from the subject, wherein the Mycobacterium polypeptide comprises the amino acid sequence set forth SEQ ID NO:
- 6.
(canceled)
A method of detecting Mycobacterium tuberculosis in a subject, comprising;
- administering to the subject an effective amount of a Mycobacterium polypeptide into the skin of the subject, wherein the Mycobacterium polypeptide comprises an amino acid sequence set forth as
- (a) the amino acid sequence set forth as SEQ ID NO:
- 6;
- or
- (b) at least nine to twenty consecutive amino acids of the amino acid sequence set forth as SEQ ID NO:
- 6, wherein the nine to twenty consecutive amino acids specifically bind major histocompatibility complex (MHC) class I;
- and
- detecting the presence of CD8+ T cells that specifically recognize the Mycobacterium polypeptide in the subject.
(canceled)
Dependencies are inferred from the claim text.