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US 2016/0103098 A1Published application
Electrophoresis buffer for extending the useful electrophoresis life of an electrophoresis gel
AssigneeDgel Electrosystem Inc.
InventorPierre SEVIGNY
Life of this patent
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Abstract
There is provided an electrolyte solution for extending useful electrophoresis life of an electrophoresis gel containing Tris(hydroxymethyl)aminomethane (TRIS), at least one zwitterion, and water. The electrolyte solution may be used in buffer systems for gel electrophoresis, such as SDS-PAGE.
Claims21
-28. (canceled)
An electrolyte solution for extending a useful electrophoresis life of an electrophoresis gel comprising:
- at least one zwitterion chosen from 2-amino-2methyl-1,3-propanediol (AMPD), N-(1,1-Dimethyl-2-hydroxyethyl)-3-amino-2-hydroxypropanesulfonic acid (AMPSO), N-Glycylglycine (Gly-Gly), 4-(2-hydroxyethyl)piperazine-1-propanesulfonic acid (EPPS or HEPPS), 3-(cyclohexylamino)-1-propanesulfonic acid (CAPS), 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (CAPSO), 2-(cyclohexylamino)ethanesulfonic acid (CHES), N,N-bis[2-hydroxyethyl]-2-aminoethanesulphonic acid (BES), (2-[2-hydroxy-1,1-bis(hydroxymethyl)ethylamino]ethanesulphonic acid) (TES), N-Tris(hydroxymethyl)methyl-3-aminopropanesulfonic acid (TAPS), 3-N-Morpholino propanesulfonic acid (MOPS), and Piperazine-N,N′-bis(2-hydroxypropanesulfonic acid) (POPSO);
- water;
- Tris(hydroxymethyl)aminomethane (TRIS), wherein the concentration of Tris(hydroxymethyl)aminomethane (TRIS) is from about 10 mM to about 500 mM;
- and
- a chelating agent selected from the group consisting of ethylene glycol tetraacetic acid (EGTA), trisodium nitrilotriacetate, hydroxyethyl ethylenediamine trisodium acetate (trisodium HEDTA), diethylene triamino pentasodium acetate or uramil disodium acetate,
- wherein the pH of the electrolyte solution is from about 8.0 to about 11.0.
3 more elements
Dependencies are inferred from the claim text.