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US 2016/0103058 A1Published application
Image forming cytometer
AssigneeChemoMetec A/S
InventorsMartin Glensbjerg, Johan Holm, Søren Kjaerulff, Frans Ejner Ravn Hansen
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Abstract
The present invention relates to methods and systems for image cytometry analysis, typically at low optical magnification, where analysis is based on detection of biological particles using UV bright field, dark field or one or more sources of excitation light. The system comprises illumination means (11, 112), a sample holder (100), a sample compartment (101), imaging means (120), collection means (121), light modulation means (122, 123), and detection means (130) with active detection elements (131).
Claims2
An image cytometer, comprising:
- a first light source configured for emitting light into a sample region,
- focusing means for forming collimated light and directing the collimated light from the first light source along an optical axis of the cytometer;
- a second light source comprising a first excitation light source configured for emitting excitation light into the sample region;
- image forming means for forming an image of at least part of the sample region on an array of detection elements, wherein
- the sample region is located between the focusing means and the array of detection elements, and wherein
- the cytometer is able to be configured to be interchanged between a bright field mode, a dark field mode and a fluorescence mode, and/or wherein
- the first light source is configured for emitting light with a wavelength less than 400 nm, and/or wherein
- the excitation light is at an incidence angle relative to the optical axis so as to provide the fluorescence mode.
5 more elements
2-123. (canceled)
Dependencies are inferred from the claim text.