All-In-One Sample Preparation Device And Method

A method of carrying out a buffer exchange, comprising:

• a. providing a sample preparation device comprising a reservoir/exchange member comprising a sample reservoir;
• a column in fluid communication with said sample reservoir, said column having a holder region and a region having a finned geometry tapering radially from a relatively thick upper portion to a relatively thin lower portion that converges in an axially extending stem having an open bottom;
• b. sealing engaging a filter device to said holder region;
• c. introducing a sample comprising a first buffer into said sample reservoir;
• d. concentrating said sample by applying centrifugal force to said sample;
• e. introducing a second buffer into said sample reservoir;
• f. applying centrifugal force to said sample again;
• and
• g. recovering the resulting sample from said filtration device.

A method of purifying a sample, comprising:

• a. providing a sample preparation device comprising a reservoir/exchange member comprising a sample reservoir;
• a column in fluid communication with said sample reservoir, said column having a holder region and a region having a finned geometry tapering radially from a relatively thick upper portion to a relatively thin lower portion that converges in an axially extending stem having an open bottom;
• b. introducing media to said column;
• c. introducing a sample to said sample reservoir;
• d. causing a fraction of said sample to bind to said media;
• e. sealing engaging a filter device to said holder region;
• and
• f. eluting said bound fraction from said media.