Event-specific detection methods
AssigneeJ.R. Simplot Company
InventorsJingsong Ye, Jeffrey W. Habig, Janet Layne, Jeffery W. Hein, Matthew G. Pence, Stephanie Hudon
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Abstract
The present disclosure concerns methods for identifying genetic material in recombinant potato plants, including in food products made from such plants. The disclosure relates to the materials, including nucleotide primers and probes, utilized in the methods set forth herein. Furthermore, the disclosure provides for non-naturally occurring nucleotide junction sequences per se that result from genetic recombination events and methods of detecting said junction sequences.
Claims39
A quantitative PCR method for detecting the presence of a plant transformation event in a nucleic acid sample, comprising:
- a) combining:
- i) a pair of forward and reverse nucleotide primers, ii) a nucleotide probe, and iii) a target nucleotide sequence from said sample comprising a non-naturally occurring nucleotide junction to be detected;
- wherein the nucleotide probe binds to the non-naturally occurring nucleotide junction, or a sequence indicative of the presence of the non-naturally occurring nucleotide junction;
- and
- b) detecting the target nucleotide sequence from said sample.
An isolated non-naturally occurring nucleic acid junction sequence sharing at least 85% sequence homology to a nucleic acid selected from the group consisting of SEQ ID NOs:
- 1-48.
An isolated non-naturally occurring nucleic acid probe sequence capable of hybridizing under stringent conditions to a nucleic acid selected from the group consisting of SEQ ID NOs:
- 1-48.
An isolated non-naturally occurring nucleic acid probe sequence sharing at least 85% sequence homology to a nucleic acid selected from the group consisting of SEQ ID NOs:
- 54, 57, 60, 63, 66, 69, 72, 75, 78, 81, 84, 87, and 90.
An isolated non-naturally occurring nucleic acid primer or probe sequence sharing at least 95% sequence homology to a nucleic acid selected from the group consisting of SEQ ID NOs:
- 52-90.
Dependencies are inferred from the claim text.