Test kits and methods for their use
AssigneePacific Edge Limited
InventorParry John Guilford
Life of this patent
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Abstract
This invention relates to test kits and methods for their use in determining the expression levels of genetic markers. Additionally, this invention relates to kits and uses thereof for the determination of ratios of genetic markers. Ratios of genetic markers can be used to assist in analysis of bladder cancer from samples of urine.
Claims26
1-66. (canceled)
A test kit, comprising:
- a forward primer and a reverse primer for hybridizing to an mRNA or cDNA transcript of insulin-like growth factor binding protein 5 (“IGFBP5”);
- a labeled probe that hybridizes to an mRNA or a cDNA transcript of said IGFBP5 between the binding locations of said forward and reverse primers of IGFBP5;
- a forward primer and a reverse primer for hybridizing to an mRNA or a cDNA oligonucleotide transcript oligonucleotide of homeobox A13 (“HOXA13”);
- a labeled probe that hybridizes to an mRNA or a cDNA transcript of said HOXA13 between the binding locations of said forward and reverse primers of HOXA13;
- a forward primer and a reverse primer for hybridizing to an mRNA or cDNA transcript of midkine (neurite growth promoting factor 2 (“MDK”);
- a labeled probe that hybridizes to an mRNA or a cDNA transcript of said MDK between the binding locations of said forward and reverse primers of MDK;
- a forward primer and a reverse primer for hybridizing to an mRNA or cDNA transcript of leukotriene B4 12-dehydrogenase (“LTB4DH”);
- and
- a labeled probe that hybridizes to an mRNA or a cDNA transcript of said LTB4DH between the binding locations of said forward and reverse primers of LTB4DH.
The kit of claim 1, at least one of said forward and reverse primers for IGFBP5, or HOXA13, or MDK, or LTB4DH comprises an exon-spanning region of a transcript of IGFBP5, or HOXA13, or MDK, or LTB4DH.
The kit of claim 60, said probe for CDC2 having the sequence of SEQ ID NO.9.
The test kit of claim 1, further comprising:
- one or more forward primer and reverse primer that bind an mRNA or a cDNA oligonucleotide transcript, and a labeled probe that can hybridizes to said transcript between said forward and reverse primer, said forward primer, said reverse primer, and said labeled probe that can hybridize to an oligonucleotide selected from the group consisting of the genetic markers in Table 1 or Table 2.
The test kit if claim 91, further comprising:
- a forward primer and a reverse primer and a labeled probe that can hybridize to an mRNA or a cDNA of one of the genetic markers tryptophanyl-tRNA synthetase (“WARS”), splicing factor, arginine/serine-rich 2 (“SFRS2”), eukaryotic translation initiation factor 4E (“EIF4E”), eukaryotic translation initiation factor 4E (also known as methenyltetrahydrofolate cyclohydrolase, formyltetrahydrofolate synthetase (“MTHFD2”), proteasome (prosome, macropain) activator subunit 2 (also known as PA28 beta) (“PSME2”), glia maturation factor, beta (“GMFB”), discs, large (Drosophila) homolog-associated protein 4 (“DLGAP4”), thymidylate synthetase (“TYMS”), cathepsin S (“CTSS”), deoxycytidine kinase (“DCK”), solute carrier family 4, sodium bicarbonate cotransporter, member 4 (“SLC4A4”), chemokine (C-X-C motif) ligand 9 (“CXCL9”), Chemokine (C-X-C motif) ligand 10 (“CXCL10”), tripartite motif-containing 25 (“TRIM25”), solute carrier family 25 (mitochondrial carrier, oxoglutarate carrier), member 11 (“SLC25A11”), complement component 1, q subcomponent binding protein (“C1QBP”), NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 9, 39 kDa (“NDUFA9”), Wolf-Hirschhorn syndrome candidate 1 (“WHSC1”), carbonic anhydrase II (“CA2”), chemokine (C-X-C motif) ligand 11 (“CXCL11”), tousled-like kinase 1 (“TLK1”), RNA binding motif protein 25 (“RBM25”), adenylate kinase 2 (“AK2”), CDC42 binding protein kinase alpha (DMPK-like) (“CDC42BPA”), SEC10-like 1 (S. cerevisiae) (“SEC10L1”), FLJ13220, KLHL24, and signal transducer and activator of transcription 1, 91 kDa (“STAT1”).
Dependencies are inferred from the claim text.