Novel ruthenium (ii) complexes, preparation and uses thereof
AssigneeCouncil of Scientific and Industrial Research
InventorsAmitava Das, Samit Chattopadhyay, Vadde Ramu, Nandaraj Taye
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Abstract
The present invention discloses novel Ruthenium (II) polypyridyl complexes, preparation and its application as DNA imaging agents.
Claims10
Novel Ruthenium (II) polypyridyl complexes of formula I
- wherein, X is selected from hydrogen or fluorine.
The novel Ruthenium (II) polypyridyl complexes of formula I, wherein, the complexes are selected from
- a) {bis-(2,2′-bpy)-(5-fluoro-1-((4′-methyl-[2,2′-bipyridin]-4-yl)methyl)pyrimidine-2,4(1H,3H)-dione)}ruthenium(II) dichloride;
- and
- b) {bis-(2,2′-bpy)-(1-((4′-methyl-[2,2′-bipyridin]-4-yl)methyl)pyrimidine-2,4(1H,3H)-dione)}ruthenium(II) dichloride.
A process for the preparation of novel Ruthenium(II) polypyridyl complexes of formula I,
- wherein, X is selected from hydrogen or fluorine
- comprising;
- a) reacting [Ru(bpy)2Cl2]2+ compound of formula 4 with the ligand of formula 3 in ethanol;
- wherein, X is selected from hydrogen or fluorine,
- b) precipitating the Ruthenium(II) polypyridyl complexes of formula I by the addition of saturated aqueous potassium hexafluorophosphate (KPF6) solution to obtain the desired complex;
- and
- c) purifying the desired complexes of formula I.
A composition comprising Ruthenium (II) polypyridyl complexes of formula I along with one or more pharmaceutical carriers for use as DNA imaging agent,
- wherein, X is selected from hydrogen or fluorine.
A method of imaging DNA in a tumor/cancer cell lines comprising
- (a) Addition of Ruthenium (II) polypyridyl complexes of formula I as imaging agent;
- and
- wherein, X is selected from hydrogen or fluorine,
- (b) subjecting/exposing the mammalian cancer cell lines to an energy source (λ=442 nm);
- and
- (c) observing the image of DNA by intracellular fluorescence intensities of the imaging agent or detecting an emission from the imaging agent using the energy source.
Ruthenium (II) polypyridyl complexes of formula I, for use as DNA imaging agents.
- wherein, X is selected from hydrogen or fluorine.
Dependencies are inferred from the claim text.