Methods for sequencing polynucleotides
AssigneeIllumina, Inc.
InventorStephen C. Macevicz
Life of this patent
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Abstract
The invention relates to methods and systems for sequencing and constructing a high resolution physical map of a polynucleotide. In accordance with the invention, nucleotide sequences are determined at the ends of restriction fragments produced by a plurality of digestions with a plurality of combinations of restriction endonucleases so that a pair of nucleotide sequences is obtained for each restriction fragment. A physical map of the polynucleotide is constructed by ordering the pairs of sequences by matching the identical sequences among the pairs.
Claims19
-17. (canceled)
A method of making a library for polynucleotide sequencing, comprising:
- (a) providing a plurality of populations of target polynucleotide fragments, each of the target polynucleotide fragments having an internal nucleotide sequence and two ends, each of the target polynucleotide fragments being inserted into a circular vector;
- and
- (b) cleaving the internal nucleotide sequence of each of the target polynucleotide fragments thereby removing the internal nucleotide sequence of each of the target polynucleotide fragments and leaving a segment of each end of the target polynucleotide fragments in the vector, thereby providing a library comprising a plurality of the segment of each end of the target polynucleotide fragments in the vector after the cleaving step.
Dependencies are inferred from the claim text.