Sequence Conversion and Signal Amplifier DNA Having Locked Nucleic Acids and Detection Methods Using Same

A composition for detecting a target nucleic acid in a sample, said composition comprising:

• a first oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a locked nucleic acid;
• a second oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence homologous to the signal DNA generation sequence of the first oligonucleotide, an endonuclease recognition site, and a sequence that is homologous to the signal DNA generation sequence of the first oligonucleotide and that comprises a locked nucleic acid.

A kit for detecting a target nucleic acid in a sample, said kit comprising:

• a first oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a locked nucleic acid;
• and
• a second oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence homologous to the signal DNA generation sequence of the first oligonucleotide, an endonuclease recognition site, and a sequence that is homologous to the signal DNA generation sequence of the first oligonucleotide and that comprises a locked nucleic acid.

A chemically modified oligonucleotide comprising, in the 5′ to 3′ direction, a first sequence that is complementary to a known signal DNA sequence, an endonuclease recognition site, and a second sequence that is complementary to the same known signal DNA sequence as the first sequence, wherein the second sequence comprises a locked nucleic acid.

A chemically modified oligonucleotide comprising, in the 5′ to 3′ direction, a first sequence that is complementary to a known signal DNA sequence, an endonuclease recognition site, and a second sequence that is complementary to the 3′-end of a target nucleic acid molecule, wherein the second sequence comprises a locked nucleic acid (LNA).

A composition for detecting a target nucleic acid in a sample, said composition comprising:

• a first oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a chemically modified nucleotide;
• a second oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence homologous to the signal DNA generation sequence of the first oligonucleotide, an endonuclease recognition site, and a sequence that is homologous to the signal DNA generation sequence of the first oligonucleotide and that comprises a chemically modified nucleotide.

A composition for detecting a target nucleic acid in a sample, said composition comprising:

• an oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a locked nucleic acid.

A kit for detecting a target nucleic acid in a sample, said kit comprising:

• an oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a locked nucleic acid.

A composition for detecting a target nucleic acid in a sample, said composition comprising:

• an oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a chemically modified nucleotide.

A kit for detecting a target nucleic acid in a sample, said kit comprising:

• an oligonucleotide comprising, in the 5′ to 3′ direction, a signal DNA generation sequence, an endonuclease recognition site, and a sequence complementary to the 3′ end of said target nucleic acid and that comprises a chemically modified nucleotide.