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US 2016/0102336 A1Published application
Fluorescent probe for detecting activity of calpain
AssigneeThe University of Tokyo
InventorsTetsuo NAGANO, Kenjiro HANAOKA, Yu KUSHIDA
Life of this patent
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Abstract
[Problem] To provide a novel fluorescent probe for detecting the activity of calpain. [Solution] A compound represented by general formula (I) or a salt thereof.
Claims20
A compound represented by general formula (I) below or a salt thereof:
- wherein
- R1 is a hydrogen atom or represents 1 to 4 monovalent substituents present on a benzene ring, which are the same or different;
- R2 is a monovalent substituent;
- R3 and R4 are, independently, a hydrogen atom or C1-6 alkyl group;
- R5 and R6 are, independently, a C1-6 alkyl group or aryl group;
- R7 and R8 are, independently, a hydrogen atom or C1-6 alkyl group;
- R9 and R10 are, independently, a hydrogen atom or C1-6 alkyl group;
- R9 or R10 optionally forms, together with R3 or R7, a 5-7-member heterocyclyl or heteroaryl containing a nitrogen atom to which R9 or R10 binds, and optionally contains 1-3 additional heteroatoms selected from the group consisting of O, N, and S as ring-constituting members;
- the heterocyclyl or heteroaryl optionally being substituted by a C1-6 alkyl, C2-6 alkenyl, C2-6 alkynyl, C6-10 aralkyl, or C6-10 aralkenyl group;
- R11 is a monovalent substituent which is to be cleaved by contact with calpain;
- and
- X is a silicon, germanium, or tin atom.
9 more elements
A compound represented by formula (4) below, or salt thereof:
A compound represented by formula (5) below, or salt thereof:
A compound represented by formula (6) below, or salt thereof:
Dependencies are inferred from the claim text.