New compact scaffold of cas9 in the type ii crispr system
AssigneeCellectis
InventorsPhilippe DUCHATEAU, Claudia BERTONATI
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Abstract
The present invention is in the field of CRISPR-Cas system for genome targeting. The present invention relates to new engineered Cas9 scaffolds and uses thereof. More particularly, the present invention relates to methods for genome targeting, cell engineering and therapeutic application. The present invention also relates to vectors, compositions and kits in which the new Cas9 scaffolds of the present invention are used.
Claims24
1-27. (canceled)
A Cas9 variant of less than 1100 amino acids, comprising RuvC and HNH domains, said domains comprising at least one RuvC motif sequence D-[I/L]-G-X-X-S-X-G-W-A or one HNH motif sequence Y-X-X-D-H-X-X-P-X-S-X-X-X-D-X-S, where X is anyone of the 20 natural amino acids.
Dependencies are inferred from the claim text.