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US 2016/0102314 A1Published application
Exogenous terminators for controlling fungal gene expression
AssigneeBoard of Regents, The University of Texas System
InventorsHal Alper, Kathleen Curran
Life of this patent
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Abstract
Provided herein are exogenous terminator sequences for use in fungi cell transcriptional termination.
Claims37
An exogenous fungi transcription terminating nucleic acid sequence comprising:
- (i) an efficiency nucleic acid sequence of the formula (TA)n1, wherein n1 is an integer from 4 to 20;
- (ii) a positioning nucleic acid sequence of the formula AAWAAA, wherein W is A or T;
- and
- (iii) a polyadenylation site nucleic acid sequence comprising the sequence (N)n4Y-An2, wherein
- N is A, C, T, or G;
- n4 is an integer from 0 to 10;
- Y is a polyadenylation site nucleotide and is C or T;
- and
- n2 is an integer from 3 to 50.
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An exogenous fungi transcription terminating nucleic acid sequence comprising
- (i) an efficiency nucleic acid sequence of the formula (TA)n1, wherein n1 is an integer from 3 to 20;
- (ii) a positioning nucleic acid sequence of the formula AAWAAA, wherein W is A or T;
- (iii) a polyadenylation site nucleic acid sequence comprising the sequence (N)n4Y-An2, wherein
- N is A, C, T, or G;
- n4 is an integer from 0 to 10;
- Y is a polyadenylation site nucleotide and is C or T;
- and
- n2 is an integer from 3 to 50;
- and
- (iv) a first linking nucleic acid sequence linking said positioning nucleic acid sequence to said efficiency nucleic acid sequence or a second linking nucleic acid sequence linking said positioning nucleic acid sequence to said polyadenylation site nucleic acid sequence, wherein said first linking nucleic acid sequence and said second linking nucleic acid sequence are independently 3 to 30 nucleotides in length and consist of less than about 35% of cytosine and guanine.
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An exogenous fungi transcription terminating nucleic acid sequence comprising
- (i) an efficiency nucleic acid sequence of the formula (TA)n1, wherein n1 is an integer from 3 to 20;
- (ii) a positioning nucleic acid sequence of the formula AAWAAA, wherein W is A or T;
- (iii) a polyadenylation site nucleic acid sequence comprising the sequence (N)n4Y-An2, wherein
- N is A, C, T, or G;
- n4 is an integer from 0 to 10;
- Y is a polyadenylation site nucleotide and is C or T;
- and
- n2 is an integer from 3 to 50.
- (iv) an upstream nucleic acid sequence 5′ to said efficiency nucleic acid sequence of the formula (T)n3, wherein n3 is an integer from 2 to 20.
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Dependencies are inferred from the claim text.