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US 2016/0102304 A1Published application
Methods and compositions for isolating small rna molecules
AssigneeLife Technologies Corporation
InventorRichard CONRAD
Life of this patent
Estimating the term…
Abstract
The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.
Claims24
A method for isolating small RNA molecules from cells comprising:
- a) lysing the cells with a lysing solution to produce a lysate;
- b) adding an alcohol solution to the lysate;
- c) applying the lysate to a solid support;
- d) eluting small RNA molecules from the solid support;
- and,
- e) using or characterizing the small RNA molecules.
3 more elements
6-20. (canceled)
(canceled)
36-48. (canceled)
A kit for isolating small RNA molecules comprising:
- a) acid phenol-chloroform;
- b) lysis/binding buffer;
- c) homogenate additive;
- d) wash solution;
- e) elution solution;
- and
- f) filter cartridges.
4 more elements
50-53. (canceled)
Dependencies are inferred from the claim text.