Protein, method for manufacturing same, and method for evaluating protein activity

A method for producing a regenerated immobilized lipase, in which a lipase activity is partially or totally regenerated, from an immobilized lipase having a reduced lipase activity, wherein the immobilized lipase is formed by immobilizing a lipase on a resin carrier, and

• wherein the method comprises a selection process, the selection process comprising:
• a step of approximating an infrared absorption band derived from a lipase appearing around 1500 to 1600 cm−1 or around 1600 to 1700 cm−1 in an infrared absorption spectrum of the immobilized lipase having a reduced lipase activity, by one or more normal distributions,
• a step of calculating an index value indicating a degree of broadening of the infrared absorption band based on the normal distributions, and
• a step of comparing the index value with a predetermined threshold to select, as an immobilized lipase in which a lipase activity is possibly regenerated, the immobilized lipase having a reduced lipase activity that has a degree of broadening of the infrared absorption band that is smaller than the threshold.

A method for evaluating a protein activity, comprising an evaluation process,

• wherein the evaluation process comprises:
• a step of approximating an infrared absorption band derived from a protein appearing around 1500 to 1600 cm−1 or around 1600 to 1700 cm−1 in an infrared absorption spectrum of the protein, by one or more normal distributions,
• a step of calculating an index value indicating a degree of broadening of the infrared absorption band based on the normal distributions, and
• a step of comparing the index value with a predetermined threshold to evaluate, as a protein having an activity, a protein having a degree of broadening of the infrared absorption band that is smaller than the threshold.