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AssigneeRoche Diagnostics Operations, Inc.
InventorsTibor Czabany, Alfred Engel, Michael Greif, Christine Jung, Christiane Luley, Sebastian Malik, Rainer Mueller, Bernd Nidetzky, Doris Ribitsch, Katharina Schmoelzer, Helmut Schwab, Harald Sobek, Bernhard Suppmann, Marco Thomann, Sabine Zitzenbacher
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The present disclosure is directed to glycosyltransferase variants having N-terminal truncation deletions. Contrary to previous findings certain truncations comprising the conserved amino acid motif (“QVWxKDS”) were found to be compatible with glycosyltransferase enzymatic activity, particularly in a human sialyltransferase (hST6Gal-I). Thus, disclosed are variants of mammalian glycosyltransferase, nucleic acids encoding the same, methods and means for recombinantly producing the variants of mammalian glycosyltransferase and use thereof, particularly for sialylating terminal acceptor groups of glycan moieties being part of glycoproteins such as immunoglobulins.
A variant mammalian glycosyltransferase, wherein the polypeptide of the variant comprises an N-terminally truncated amino acid sequence of the wild-type mammalian glycosyltransferase, the truncation comprising the amino acid sequence motif of SEQ ID NO:2, and wherein the variant exhibits glycosyltransferase activity.
Dependencies are inferred from the claim text.