Endocardium-derived adult stem cells and method for producing same
AssigneeSeoul National University Hospital
InventorsHyo-Soo KIM, Han-Mo YANG, Ju-Young KIM, Hyun-Jai CHO
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Abstract
The present invention relates to endocardium-derived adult stem cells obtained by culturing peripheral blood mononuclear cells (PBMCs) separated from peripheral blood, and a cell therapeutic agent for treating cardiovascular diseases containing the same as an active ingredient. The adult stem cells, according to the present invention, have an origin that is the endocardium and strong blood vessel formation, and is thus remarkably useful for treating cardiovascular diseases such as ischemia, myocardial infarction, and the like.
Claims8
An endocardium-derived adult stem cell that is obtained when peripheral blood mononuclear cells (PBMCs) separated from peripheral blood are suspended in an EGM-2MV (Microvascular Endothelial Cell Growth Media-2) medium and seeded, and T cells are removed and a culture is performed while the medium is exchanged daily for 5 to 8 days.
A method of preparing endocardium-derived adult stein cells, comprising:
- (a) a step in which peripheral blood mononuclear cells (PBMCs) are separated from peripheral blood and then suspended in an EGM-2MV (Microvascular Endothelial Cell Growth Media-2) medium and then seeded;
- and
- (b) a step in which the medium is exchanged daily for 5 to 8 days after the seeding, T cells are removed and a culture is performed.
Dependencies are inferred from the claim text.