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US 2016/0102293 A1Published application
Device and method of 3-dimensionally generating in vitro blood vessels
AssigneeSnu R&Db Foundation
InventorsNoo Li Jeon, Ju Hun Yeon, Qing Ping Hu, Sudong Kim, Hyun Jae Lee
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Abstract
Apparatuses, systems, and methods for generating concentration gradients of soluble molecules are disclosed herein. Devices and methods for generating in vitro blood vessels are also disclosed.
Claims5
A method of generating a blood vessel comprising:
- a) injecting i) an gel and/or an extracellular matrix (ECM) and ii) a blood vessel-forming cell into a blood vessel-forming channel of a blood vessel-generating device comprising a) a sink channel in fluid communication with a sink inlet, b) a source channel in fluid communication with a source inlet substantially parallel to the sink channel, c) the blood vessel-forming channel in fluid communication with a blood vessel-forming channel inlet, disposed in contact with the sides of the sink channel and the source channel between the sink channel and the source channel, substantially parallel to the source channel and the sink channel, d) a first culture channel in fluid communication with a first culture channel inlet, disposed in contact with the other side of the sink channel, substantially parallel to the sink channel and e) a second culture channel in fluid communication with a second culture channel inlet, disposed in contact with the other side of the source channel, substantially parallel to the source channel, wherein a plurality of microstructures configured to allow interaction between biochemical materials included in each channel are arranged in the interfaces of the respective adjacent channels at set intervals, and wherein the sink channel is disposed between the blood vessel-forming channel and the first culture channel, and the source channel is disposed between the blood vessel-forming channel and the second culture channel;
- b) injecting the ECM and a co-culturing cell into the first culture channel and/or the second culture channel of the blood vessel-generating device;
- and
- c) injecting an angiogenesis factor and/or a cell culture medium into the sink channel and/or the source channel of the blood vessel-generating device, and culturing the blood vessel-forming cell and the co-culturing cell;
- and
- d) interacting the blood vessel-forming cell with the co-culturing cell via paracrine signaling.
3 more elements
A method of generating a blood vessel comprising:
- a) injecting a gel and/or an extracellular matrix (ECM) into the blood vessel-forming channel of the blood vessel-generating device comprising a) a sink channel in fluid communication with a sink inlet, b) a source channel in fluid communication with a source inlet substantially parallel to the sink channel, c) a blood vessel-forming channel in fluid communication with a blood vessel-forming channel inlet, disposed in contact with the sides of the sink channel and the source channel between the sink channel and the source channel, substantially parallel to the source channel and the sink channel, d) a first culture channel in fluid communication with a first culture channel inlet, disposed in contact with the other side of the sink channel, substantially parallel to the sink channel and e) a second culture channel in fluid communication with a second culture channel inlet, disposed in contact with the other side of the source channel, substantially parallel to the source channel, wherein a plurality of microstructures configured to allow interaction between biochemical materials included in each channel are arranged in the interfaces of the respective adjacent channels at set intervals, and wherein the sink channel is disposed between the blood vessel-forming channel and the first culture channel, and the source channel is disposed between the blood vessel-forming channel and the second culture channel;
- b) injecting a blood vessel-forming cell into the sink channel and/or the source channel of the blood vessel-generating device, and attaching the blood vessel-forming cell to the gel and/or the ECM exposed between the microstructures arranged in the sides of the blood vessel-forming channel;
- c) injecting the ECM and a co-culturing cell into the first culture channel and/or the second culture channel of the blood vessel-generating device;
- and
- d) injecting an angiogenesis factor and a cell culture medium into the sink channel and/or the source channel of the blood vessel-generating device, and culturing the blood vessel-forming cell and the co-culturing cell;
- e) interacting the blood vessel-forming cell with the co-culturing cell via paracrine signaling.
3 more elements
Dependencies are inferred from the claim text.