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US 2016/0102286 A1Published application
Methods For The Cryopreservation Of Mammalian Cells
AssigneeThe General Hospital Corporation
InventorsMehmet Toner, Heidi Y. Elmoazzen, Thomas L. Toth, Yunseok Heo, Ho-Joon Lee, Nilay Chakraborty
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Abstract
Manufactured capillary tubes can include: a tubular member; and a coating applied partially covering the tubular member, the coating defining a window where the tubular member is free of the coating. Kits containing and methods related to the capillary tubes can be used for the cryopreservation of cells.
Claims20
(canceled)
A method for cryopreserving a cell, the method comprising:
- (a) suspending a cell in a vitrification solution comprising one or more cryoprotective agents, wherein the concentration of the combination of all cryoprotective agents in the vitrification solution is less than or equal to 4 M;
- (b) placing the cell in the vitrification solution into a fused silica microcapillary tube having an outer coating on the surface of the microcapillary tube, a wall thickness of less than or equal to about 20 μm, and an inner diameter of less than or equal to about 200 μm, wherein the coating has been removed on at least a portion of the microcapillary tube to define a window;
- and
- (c) cooling said cell in the vitrification solution in the microcapillary tube by plunging the microcapillary tube into slush nitrogen for a time sufficient to cause vitrification of the cell in the absence of ice formation within the cell.
1 more element
Dependencies are inferred from the claim text.