System for incubating microfluidic droplets and method for producing homogeneous incubation conditions in a droplet incubation unit
AssigneeLeibniz-Institut FüR Naturstoff-Forschung Und Infektionsbiologie—Hans-KnöLl-Institut
InventorsMiguel TOVAR, Lisa MAHLER, Emerson ZANG, Martin ROTH
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Abstract
A system for incubating microfluidic droplets includes a droplet incubator, a gas exchanger and a conveyor. A method provides homogeneous incubation conditions in a droplet incubator by targeted introduction of gases and/or gas mixtures into a carrier fluid.
Claims15
System for incubating microfluidic droplets, comprising a droplet incubator, a gas exchanger and a conveyor,
- wherein in the droplet incubator, microfluidic droplets are present, which comprise cell suspensions and/or dissolved reagents, and the droplet incubator is connected by a flow-through conduit to a gas exchanger, wherein the flow-through conduit forms a circuit that conducts a carrier fluid which comprises gas or gas mixtures, and the conveyor leads the carrier fluid continuously through the droplet incubator and the gas exchanger.
Method for providing homogeneous incubation conditions in a droplet incubator by targeted introduction of gases or gas mixtures into a carrier fluid, the method comprising the following steps:
- a) generating a cyclic and/or continuous flow of the carrier fluid through a closed circuit formed by a flow-through conduit, a droplet incubator and a gas exchanger,
- b) exchanging at least a portion of the gases or gas mixtures dissolved in the carrier fluid during a residence time of the carrier fluid in the gas exchanger, and
- c) exchanging at least a portion of the gases or gas mixtures dissolved in the carrier fluid, during the flow of the carrier fluid through the droplet incubator.
Dependencies are inferred from the claim text.