US 2016/0102136 A1Published application

Single-domain vhh antibodies directed to norovirus gi.1 and gii.4 and their use

AssigneesThe United States of America, as represented by the Secretary, Dept. of Health and Human Services, Instituto Nacional De Tecnologia Agropecuaria

InventorsKarin Bok, Lorena Laura Garaicoechea, Viviana Parreno, Andrea Pamela Aguilar, Marina Bok, Lisbeth Kim Green, Stanislav Vladimirovich Sosnovtsev

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Abstract

Isolated VHH monoclonal antibodies are disclosed that specifically bind to a Norovirus polypeptide. In some embodiments, the Norovirus is a Genogroup I Norovirus or a Genogroup II Norovirus. In other embodiments, the Norovirus is Norwalk or MD2004 virus. In some embodiments, the monoclonal antibodies specifically bind VP1. Also disclosed are compositions including the disclosed antibodies, nucleic acids encoding these antibodies, expression vectors including the nucleic acids, and isolated host cells that express the nucleic acids. The antibodies and compositions disclosed herein can be used for detecting the presence of a Norovirus in a biological sample, or detecting a Norovirus infection. Also disclosed are methods of treating and/or preventing a NoV infection.

Claims31

Dependent claims

An isolated VHH monoclonal antibody comprising a heavy chain domain, wherein the heavy chain variable domain comprises a heavy chain complementarity determining region (HCDR)1, an HCDR2 and an HCDR3, wherein the CDR3 comprises the amino acid sequence set forth as one of:

a) amino acids 96-109 of SEQ ID NO:
3;
b) amino acids 96-109 of SEQ ID NO:
2;
c) amino acids 96-109 of SEQ ID NO:
1;
d) amino acids 96-109 of SEQ ID NO:
4;
e) amino acids 97-110 of SEQ ID NO:
5;
f) amino acids 97-111 of SEQ ID NO:
6;
g) amino acids 97-111 of SEQ ID NO:
7;
h) amino acids 97-111 of SEQ ID NO:
8;
i) amino acids 96-112 of SEQ ID NO:
9;
j) amino acids 96-112 of SEQ ID NO:
10;
k) amino acids 96-114 of SEQ ID NO:
11;
l) amino acids 96-112 of SEQ ID NO:
12;
m) amino acids 97-113 of SEQ ID NO:
13;
n) amino acids 97-113 of SEQ ID NO:
14;
o) amino acids 97-114 of SEQ ID NO:
15;
p) amino acids 96-107 of SEQ ID NO:
16;
q) amino acids 97-113 of SEQ ID NO:
17;
r) amino acids 97-114 of SEQ ID NO:
18;
s) amino acids 97-113 of SEQ ID NO:
19;
t) amino acids 96-111 of SEQ ID NO:
20;
u) amino acids 96-102 of SEQ ID NO:
21;
v) amino acids 96-110 of SEQ ID NO:
22;
w) amino acids 95-104 of SEQ ID NO:
23;
x) amino acids 96-107 of SEQ ID NO:
24;
y) amino acids 100-110 of SEQ ID NO:
25;
z) amino acids 96-117 of SEQ ID NO:
26;
aa) amino acids 97-108 of SEQ ID NO:
27;
bb) amino acids 96-112of SEQ ID NO:
28;
cc) amino acids 97-115 of SEQ ID NO:
29;
dd) amino acids 97-121 of SEQ ID NO:
30, and wherein the VHH monoclonal antibody specifically binds a Norovirus polypeptide.

57 more elements

Dependencies are inferred from the claim text.