Consumable Cryopreserved Cells Transiently Overexpressing Gene(s) Encoding Drug Transporter Protein(s) and/or Drug Metabolizing Enzyme(s)
AssigneeCorning Incorporated
InventorsNa Li, Jie Wang, Christopher J. Patten
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Abstract
The present invention discloses cryopreserved recombinant cells for screening drug candidates that transiently overexpress one or more drug transporter proteins and/or drug metabolizing enzymes. Advantageously, such cells provide a cost-efficient consumable product that streamlines the process of screening whether drug candidates are substrates or inhibitors of drug transporter proteins and/or drug metabolizing enzymes.
Claims20
A cryopreserved recombinant cell comprising one or more transiently overexpressed genes encoding a drug transporter protein, wherein activity of the drug transporter protein is detectable in a population of the cryopreserved recombinant cell following thaw from cryopreservation.
A process of preparing transiently transfected recombinant cells which transiently overexpress one or more genes encoding a drug transporter protein, comprising transiently transfecting cells with one or more genes encoding a drug transporter protein and cryopreserving the transiently transfected recombinant cells within 48 hrs of transfection.
Dependencies are inferred from the claim text.