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US 2016/0102125 A1Published application
Expression systems and associated methods
AssigneeUtah State University
InventorsRandolph V. Lewis, Dong Chen, Justin A. Jones, Sreevidhya T. Krishnaji, Paula F. Oliveira, Gargi Bhattacharyya
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Abstract
A method of producing synthetic spider silk, including: transforming Escherichia coli with an expression vector; fermenting the transformed E. coli in a culture medium; inducing spider silk protein expression in the cultured E. coli; extracting the spider silk; and purifying the spider silk. Related vectors and genetically modified cells are also disclosed.
Claims20
A method of producing a synthetic spider silk, comprising:
- transforming one or more bacterial cells with an expression vector system comprising a first antibiotic resistance gene, a spider silk protein-encoding open reading frame, and a transfer RNA gene, to generate transformed bacterial cells;
- fermenting the transformed bacterial cells in a culture medium;
- inducing spider silk protein expression in the cultured E. coli with an inducer;
- and
- purifying the synthetic spider silk protein.
2 more elements
A DNA vector system for expressing spider silk proteins in bacteria comprising:
- a first antibiotic resistance gene, a spider silk protein-encoding open reading frame, and a transfer RNA gene.
Dependencies are inferred from the claim text.